Journal: American Journal of Nuclear Medicine and Molecular Imaging
Article Title: 68 Ga-labeled TMTP1 radiotracer for PET imaging of cervical cancer
doi: 10.62347/NFDH6303
Figure Lengend Snippet: Cell migration and invasion assays as well as cellular affinity of 68Ga-DOTA-TMTP1. A and B. Representative micrographs and quantification of wound healing assay for C33A and Hela tumor cells at 0 h and 24 h (Bar =100 μm). C and D. Transwell invasion assay for C33A and Hela tumor cells (Bar =20 μm). Quantification of migration and invasion assays were calculated by three independent experiments. E. Cell uptake assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. F. Cell efflux assay of 68Ga-DOTA-TMTP1 in End1, C33A and Hela cells. Data are expressed as mean ± SD. G and H. Cell uptake blocking experiments in Hela and C-33A cells. **P < 0.01, ***P < 0.001.
Article Snippet: The human cervical epithelial cell line End1 and the human cervical cancer cell lines C-33A and Hela were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Migration, Wound Healing Assay, Transwell Invasion Assay, Blocking Assay